Gria1 (phospho S845) polyclonal antibody
产品名称： Gria1 (phospho S845) polyclonal antibody
英文名称： Gria1 (phospho S845) polyclonal antibody
产品编号： PAB9662
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Gria1.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding S845 of rat Gria1.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 100
- Reactivity:
- Human, Mouse, Primates, Rat
- Form:
- Liquid
- Purification:
- Affinity purification
- Storage Buffer:
- In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Postsynaptic protein phosphorylation and LTP.
Soderling TR, Derkach VA.Trends Neurosci. 2000 Feb;23(2):75-80.
- 2.
- Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin-dependent kinase II.
Mammen AL, Kameyama K, Roche KW, Huganir RL.J Biol Chem. 1997 Dec 19;272(51):32528-33.
- 3.
- Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit.
Roche KW, O'Brien RJ, Mammen AL, Bernhardt J, Huganir RL.Neuron. 1996 Jun;16(6):1179-88.
- Applications
- Western Blot (Tissue lysate)
- Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k Gria1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Gria1 (phospho S845) polyclonal antibody (Cat # PAB9662). The immunolabeling is completely eliminated by treatment with lambda-Ptase.