GDF15 monoclonal antibody, clone 7C12.B3.F2
产品名称: GDF15 monoclonal antibody, clone 7C12.B3.F2
英文名称: GDF15 monoclonal antibody, clone 7C12.B3.F2
产品编号: MAB8162
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against synthetic peptide of GDF15 .
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus end of human GDF15 .
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:150000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Changes in gene expression contribute to cancer prevention by COX inhibitors.
Baek SJ, Eling TE.Prog Lipid Res. 2006 Jan;45(1):1-16. Epub 2005 Nov 21.
- 2.
- H6D polymorphism in macrophage-inhibitory cytokine-1 gene associated with prostate cancer.
Lindmark F, Zheng SL, Wiklund F, Bensen J, Balter KA, Chang B, Hedelin M, Clark J, Stattin P, Meyers DA, Adami HO, Isaacs W, Gronberg H, Xu J.J Natl Cancer Inst. 2004 Aug 18;96(16):1248-54.
- Applications
- Western Blot
- Western blot using GDF15 monoclonal antibody, clone 7C12.B3.F2 (Cat # MAB8162).
The blot shows detection of recombinant GDF15 protein present in Pichia pastoris whole cell lysates.
Lane 1, yeast cell lysate expressing GDF15 H variant with SUMO expression tag at 36 KDa
Lane 2, yeast cell lysate expressing GDF15 D variant with SUMO expression tag at 36 KDa.
Lane 3, yeast cell lysate expressing GDF15 H variant.
Lane 4, yeast cell lysate expressing GDF15 D variant.
All lysates were run under reducing conditions. Primary antibody was used at a 1 : 1,000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.
- Entrez GeneID:
- 9518
- Gene Name:
- GDF15
- Gene Alias:
- GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB
- Gene Description:
- growth differentiation factor 15
- Omim ID:
- 605312
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Bone morphogenetic proteins (e.g., BMP5; MIM 112265) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM
- Other Designations:
- NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1,PTGF-beta,prostate differentiation factor
- Related Disease
- Adenoma
- Adenomatous Polyps
- Alzheimer Disease
- Alzheimer disease
- Carcinoma
- Cardiovascular Diseases
- Colorectal Neoplasms
- Diabetes Complications
- Diabetes Mellitus, Type 2
- Edema
- Genetic Predisposition to Disease
- Hypertension
- Hypertrophy, Left Ventricular
- Lung Neoplasms
- Metabolic Syndrome X
- Neoplasm Metastasis
- Neoplasms
- Osteoporosis
- Prostate cancer