IRS1 (phospho S312) polyclonal antibody
产品名称： IRS1 (phospho S312) polyclonal antibody
英文名称： IRS1 (phospho S312) polyclonal antibody
产品编号： PAB12627
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
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- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of IRS1.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding S312 of human IRS1.
- Sequence:
- ATSP
- Host:
- Rabbit
- Theoretical MW (kDa):
- 165
- Reactivity:
- Human, Mouse, Rat
- Specificity:
- This antibody recognizes ~165 KDa of human IRS1 (pSer312).
- Form:
- Liquid
- Storage Buffer:
- In TBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).
Li Y, Soos TJ, Li X, Wu J, Degennaro M, Sun X, Littman DR, Birnbaum MJ, Polakiewicz RD.J Biol Chem. 2004 Oct 29;279(44):45304-7. Epub 2004 Sep 10.
- Applications
- Immunoprecipitation
- The cell lysate derived from insulin stimulated CHO was immunoprecipitated by IRS1 (phospho S312) polyclonal antibody (Cat # PAB12627), then immunoprobed by the same antibody at 1 : 500 (Lane 1). Lane 2 is a negative control.
- Dot Blot (Peptide)
- Dot Blot : 1 ug peptide was blot onto NC membrane.
A : IRS1 (pS312).
B : IRS1 (non phosphorylated).
C : Non-related Phosphopeptide.
Followed by rabbit antibodies incubation at a 1 : 1000 dilution.
1. IRS1 (phospho S312) polyclonal antibody (Cat # PAB12627).
2. IRS1 polyclonal antibody (Cat # PAB12695).
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